Recombinant Trypsin (USP)

Catalog Number:博坊在线
CAS:9002-07-7
EC:3.4.21.4
Source:Porcine trypsin, expressed inE.coli

1.DESCRIPTION
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7.0-9.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF , aprotinin, soybean trypsin inhibitor and by metal chelating agents, e.g. EDTA. It is typically isolated from the pancreas of animals. Animal-derived materials are now subject to more stringent regulations, as authorities strive to ensure safer biologics and pharmaceuticals, such as in insulin and vaccine production.
Recombinant porcine trypsin is a genetically engineered protein expressed in E.coli and purified by HPLC. It is manufactured under NSF ISO 9001-2008 and in accordance to good manufacturing practice (GMP) guidelines. No animal derived products are used in the fermentation, purification, and final formulation.

2.ADVANTAGES
（1）Animal origin free:No animal-derived material is used during production.
（2）High purity:
1) Recombinant porcine trypsin provides increased specific activity no less than 3800 USP units/mg pro.
2) Reduce production costs while improving manufacturing progresses.

3.MAIN FEATURES

UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

5.RECOMMEND USAGE
Prepare 1-10mg/ml 博坊在线 with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.

6. STORAGE INSTRUCTIONS
Recombinant trypsin solution should be stored under -70℃. It is stable within 24 months.

7. RELATED PRODUCTS
Recombinant trypsin (Lyophilized powder), Recombinant Human Trypsin, Sequencing Grade Modified Trypsin.