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Catalog Number:SRCPB01
CAS:9025-24-5
EC:3.4.17.2
Storage temperature:
2-8℃
S
ource:
Sequencing grade recombinant carboxypeptidase B is a genetically engineered protein expressed in
E.coli
Protein Sequence:The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B
1.
DESCRIPTION
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA.
2.
MAIN FEATURES
Source
|
Recombinant
E.
c
oli
|
Appearance
|
White or off white, or yellowish powder
|
Specific activity
|
≥200 units/mg pro.
|
SDS-PAGE
|
Single major band
|
Molecular Weight(
SDS-PAGE
)
|
33.8±3.4 kDa
|
Purity(HPLC)
|
≥ 95%
|
3.
RECOMMENDUSAGE
Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Sequencing grade carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container.It is stable within 24 months.
After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃.
Avoid repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
-
Sequence grade recombinant protein: high specificity,good stability.
-
Animal origin free: Sequencing grade carboxypeptidase B is no exogenous virus contamination,and any animal origin material is not used in the production process.
-
Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
-
High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
-
Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
-
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
-
Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
6.PRODUCT USAGE
1) Determination of protein C-terminal basic amino acids such as arginine, lysine and histidine.
2) detection of antibody basic peak.
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