Recombinant Trypsin
Catalog Number:
RPT0201
CAS: 9002-07-7
EC: 3.4.21.4
Storage temperature:
2-8℃
Source: Recombinant trypsin is a genetically engineered protein expressed in
E.coli
Protein Sequence:The amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin
1.
DESCRIPTION
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. 博坊在线 recombinant trypsin is a genetically engineered protein expressed in
E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .博坊在线 can replace native trypsin for using in a variety of biotechnological processes. The M.W. of recombinant trypsin is 24KD,and the optimum pH is pH 7.0-11.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.
2.
MAIN FEATURES
|
Source
|
Recombinant
E.
c
oli
|
|
Appearance
|
White or off white, or yellowish powder
|
|
Specific activity
|
≥3800 USP units/mg pro.
|
|
Purity(
SDS-PAGE
)
|
Single major band
|
|
Molecular Weight(
SDS-PAGE
)
|
24.0±2.4 kDa
|
UNIT DEFINITION
：One USP unit of trypsin activity will produce a Delta A253
of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
3.
R
E
COMMENDUSAGE
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: 博坊在线 lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃.
It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport:
The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
-
Animal origin free: recombinant trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
-
Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
-
High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
-
Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
-
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
-
Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
6.PRODUCT USAGE
The amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .博坊在线 can replace native trypsin for using in a variety of biotechnological processes,such as in insulin and vaccine production.
7.RELATED PRODUCTS
Recombinant carboxypeptidase B
Sequencing grade trypsin
Recombinant human trypsin
Recombinant enterokinase
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